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| IAC 2004: New humic acid derivative as potent inhibitors of HIV-1 replication |
2004-07-23 11:00:00 <PWHA-NET> |
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IAC 2004: New humic acid derivative as potent inhibitors of HIV-1 replication
Abstract Number: A10360
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Author: G Kornilaeva1, A Bercovich2, T Pavlova1, E Karamov1
1Ivanovsky institute of virology, RAMS, Moscow, Russian Federation; 2Ligfarm, Moscow, Russian Federation
Background:
The anti-HIV-1 activity of the new humic acid derivative has been determined in vitro, and the serial passages at low concentrations have been performed to investigate the emergence of resistance to this drug candidate.
Methods:
The antiviral efficiency of the humic acid derivative was tested against HIV-1 T-tropic laboratory strain and HIV-1 M-tropic AZT-resistant wild-type strain. CEM cells were infected and cultivated in the presence of compound at the concentrations 250-0.0025 µg/ml. The level of virus reproduction in infected cells was detected with p24 HIV-1 antigen ELISA detection system. The serial virus passages were performed at the concentration - 0.0025 µg/ml of compound. The virus generation of each passage was analyzed for HIV-1 p24 antigen and the infectious activity.
Results:
The IC50 values of the humic acid derivative against HIV-1 T-tropic laboratory strain and HIV-1 M-tropic AZT-resistant wild-type strain were 0.85 and 3.5 µg/ml respectively. The combination of humic acid derivative and AZT intensifies their anti virus activity in 30-100 times. The determined p24 HIV-1 antigen value of the first passage virus generation at the presence of 0.0025 µg/ml of compound was identical to that of the control while the infectious activity approached to zero. The virus generations of second and third passages possessed the low values of p24 HIV-1 antigen and the undetectable infectious activity.
Conclusions:
The new acid humic derivative is effective inhibitor of various HIV-1 variants with high selectivity indexes (5500-3000). According to our date this compound disorder HIV-1 replication and yield the defective unifectious virions.
Copyright © IAS
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